Enzyme-linked immunosorbent assay (ELISA) Test: Price & Results

Enzyme-linked immunosorbent assay (ELISA) Test: Price & Results

ELISA Test is a blood test to identify diseases by detecting antibodies. It's helpful in various fields like food safety, environmental monitoring, and research.

 

ELISA Test

Here are the basic details of the ELISA Test.

Also Known As Enzyme-Linked Immunosorbent Assay
Type Blood Test
Purpose Detects antibodies in various diseases, research, and more
Sample Type Serum
Preparation No
Fasting Not required
Gender Unisex
Age Group All
Normal Value Negative
Reporting Time 24 hours
Cost 500-1500 INR*

*Price range may vary as per location, lab type, and procedure of lab test.

 

ELISA Test Means

ELISA Test, short for Enzyme-Linked Immunosorbent Assay, detects antibodies in blood samples.

  • It's a vital diagnostic tool for infectious diseases like HIV and Lyme disease.
  • ELISA testing is non-invasive and usually painless.
  • Results may require confirmation due to the possibility of false positives or negatives.
  • It's widely used in healthcare, research, and diagnostics.

 

ELISA Test Purpose

The purpose of the ELISA Test is to:

  • ELISA Test identifies antibodies in blood to diagnose diseases like HIV and Lyme disease.
  • Essential for early disease detection.
  • Used in research to analyze antibody-antigen interactions.
  • Screening for various health conditions.
  • Determines immune responses and aids in disease management.

ELISA Test_ Price, Normal Range, Result, Purpose, Meaning and Interpretation - Drlogy Test

 

ELISA Test Preparation

Here is the basic preparation for the ELISA Test.

 

Before Test

  • No fasting typically required.
  • Discuss concerns with healthcare provider.
  • Understand the purpose of the test.

 

During Test

  • Blood sample drawn, mild discomfort.
  • Needle insertion, tourniquet, and sample collection.
  • Maintain pressure at the needle site after removal.

 

After Test

  • Minimal post-test recovery.
  • May experience slight bruising or tenderness.
  • Await results and follow up as needed.

 

ELISA Test Procedure

Here is the basic ELISA Test Procedure.

  • Blood sample collection.
  • Arm cleaned and tourniquet applied.
  • Needle inserted into vein.
  • Blood drawn for analysis.
  • Sample sent to a laboratory.
  • Mixed with specific antigen.
  • Enzyme added to observe reaction.
  • Change in color indicates antibody presence.
  • Results analyzed by technicians.
  • Interpretation based on specific condition tested.

 

ELISA Test Result

  • Positive result: Presence of specific antibodies.
  • Negative result: No antibodies detected.
  • May require follow-up or confirmation testing for accuracy.

 

ELISA Test Normal Range

Here is the normal range of the ELISA Test.

Parameter Normal Range
Detection Range (for most tests) 0.01 ng to 0.1 ng (or 0.1 to 1 fmole)
Negative Control (absorbance at 490 nm) < 0.1
Positive Control (absorbance at 490 nm) > 0.15

 

ELISA Test Interpretation

Here is the Interpretation of the ELISA Test.

Result Interpretation
Positive Presence of antibodies or antigens above the cutoff value. Suggestive of infection or exposure.
Negative Antibodies or antigens below the cutoff value. Indicates no or insufficient presence.
Borderline Close to the cutoff value. May require retesting or further evaluation.
Invalid Test didn't produce reliable results. Repeat test recommended.

 

ELISA Test Positive

Here are some possible causes of positive ELISA test.

Causes Effects
HIV infection Indicates the presence of HIV antibodies.
Lyme disease exposure Suggests prior exposure to Lyme disease bacteria.
Syphilis infection Signifies the presence of syphilis antibodies.
Exposure to specific virus Shows exposure or infection with the indicated virus.

ELISA tests detect antibodies or antigens in blood, aiding in diagnosing various diseases and conditions.

 

ELISA Test Negative

Here are some possible causes of negative ELISA test.

Causes Effects
Early-stage infection May not have produced detectable antibodies yet.
Immune response threshold Antibody levels are below the test's detection limit.
No exposure to the antigen No antibodies present, indicating no prior exposure.
Test limitations False negatives can occur due to test sensitivity.

ELISA test detects antibodies, with positive results indicating exposure or infection, while negatives suggest no or early exposure.

 

Specimen Requirements For ELISA Test

Here are the specimen requirements for the ELISA Test.

Specimen

Blood or Saliva

Volume

1 ml

Container

Red-top tube or gel-barrier tube

Storage

Room temperature

Sample Stability

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x4

Causes for Rejection

Microbially-contaminated specimen; heat-treated specimen; specimens containing particulate matter; gross hemolysis; lipemia or icterus

 

ELISA Test is Safe?

ELISA tests are generally safe and non-invasive.

  • Minimal discomfort during blood sample collection.
  • Rare risks include infection, fainting, bruising, or bleeding.
  • Safety also depends on the skills of the healthcare provider performing the test.
  • Discuss any concerns with your healthcare provider before the test.

 

When Do You Get ELISA Test Results? 

  • ELISA test results typically take one day to be processed.
  • The specific timeframe can vary depending on the laboratory and test type.
  • Your healthcare provider will inform you when to expect the results.
  • In some cases, more complex tests may take longer to provide results.
  • It's essential to follow up with your healthcare provider for result interpretation and any necessary actions.

 

ELISA Test Limitation

Here is the basic limitation of ELISA Test.

  • ELISA can yield false positives or negatives, impacting diagnostic accuracy.
  • It may detect related antigens, leading to misinterpretation.
  • Not suitable for all conditions.
  • Proper technique and training are necessary for reliable results.
  • Sensitivity varies depending on the test and the disease being evaluated.

 

ELISA Test Risk Factors

Here are some of the risk factors of the ELISA Test.

  • Blood collection carries a small chance of infection at the puncture site.
  • Some individuals may experience dizziness during or after the test.
  • Bruising at the puncture site is common, as is mild bleeding.
  • Uncommon, but some may experience more significant bleeding.
  • While generally well-tolerated, some people may find the procedure uncomfortable.

 

Doctor Recommendations After ELISA Test Result

 Here are the Doctor's recommendations or consult a specialist after the ELISA Test.

Result Doctor to Visit Reason
Positive Infectious Disease Confirm diagnosis.
Negative Consulting Physician Further evaluation.

 

ELISA Test Price

Here are the estimated ELISA Test Prices in India with different top cities:

City

Price Range (INR)*

Mumbai

500-1500

New Delhi

550-1500

Bangalore

500-1500

Hyderabad

550-1500

Kolkata

500-1500

Pune

550-1500

Lucknow

500-1500

Noida

550-1500

Surat

550-1500

Gurugram

500-1500

Patna

550-1500

Chennai

500-1500

Jaipur

550-1500

Ahmedabad

500-1500

*Prices are approximate and vary depending on a specific laboratory or healthcare facility.

 

Summary

Overall, ELISA Test, a versatile diagnostic tool, detects antibodies or antigens in blood samples, aiding in disease diagnosis and research. Also check Drlogy Test for detailed information about all medical tests for patients, doctors, scholers and medical students.

 

Reference

  • ELISA - Wikipedia [1].
  • Enzyme Linked Immunosorbent Assay - NIH [2].

 

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FAQ

What is an ELISA test?

An Enzyme-Linked Immunosorbent Assay (ELISA) test is a highly sensitive laboratory technique used to detect and measure the presence of specific substances like antibodies, antigens, proteins, or hormones in biological samples. ELISA tests are widely used in medical diagnostics and research to diagnose diseases, monitor treatment outcomes, and study various biomarkers.

What are the types of ELISA tests?

ELISA tests come in several forms, including direct, indirect, sandwich, and competitive ELISAs. Direct ELISA detects antigens directly, while indirect ELISA uses a secondary antibody to detect antibodies. Sandwich ELISA is used to detect specific antigens in complex samples, and competitive ELISA measures the competition between a labeled antigen and an unlabeled one for a limited number of antibodies. Each type has its advantages and is chosen based on the specific application's requirements.

What is the purpose of an ELISA test?

ELISA tests have various applications, such as diagnosing infectious diseases, measuring hormone levels, monitoring autoimmune disorders, detecting allergies, and assessing the presence of specific proteins in biological samples. They are also used in drug development and research to study various biomarkers and drug interactions. ELISA is a versatile tool in the healthcare and research fields due to its sensitivity and specificity.

What are the advantages of ELISA tests?

ELISA tests offer several advantages, including high sensitivity and specificity, the ability to detect multiple analytes simultaneously, quantification of target molecules, and the potential for automation. They are also relatively cost-effective and suitable for a wide range of applications in both clinical and research settings. These attributes make ELISA a valuable tool in diagnosing diseases, monitoring treatment efficacy, and conducting biomedical research.

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