ELISA Test is a blood test to identify diseases by detecting antibodies. It's helpful in various fields like food safety, environmental monitoring, and research.
Here are the basic details of the ELISA Test.
Also Known As | Enzyme-Linked Immunosorbent Assay |
Type | Blood Test |
Purpose | Detects antibodies in various diseases, research, and more |
Sample Type | Serum |
Preparation | No |
Fasting | Not required |
Gender | Unisex |
Age Group | All |
Normal Value | Negative |
Reporting Time | 24 hours |
Cost | 500-1500 INR* |
*Price range may vary as per location, lab type, and procedure of lab test.
ELISA Test, short for Enzyme-Linked Immunosorbent Assay, detects antibodies in blood samples.
The purpose of the ELISA Test is to:
Here is the basic preparation for the ELISA Test.
Here is the basic ELISA Test Procedure.
Here is the normal range of the ELISA Test.
Parameter | Normal Range |
---|---|
Detection Range (for most tests) | 0.01 ng to 0.1 ng (or 0.1 to 1 fmole) |
Negative Control (absorbance at 490 nm) | < 0.1 |
Positive Control (absorbance at 490 nm) | > 0.15 |
Here is the Interpretation of the ELISA Test.
Result | Interpretation |
---|---|
Positive | Presence of antibodies or antigens above the cutoff value. Suggestive of infection or exposure. |
Negative | Antibodies or antigens below the cutoff value. Indicates no or insufficient presence. |
Borderline | Close to the cutoff value. May require retesting or further evaluation. |
Invalid | Test didn't produce reliable results. Repeat test recommended. |
Here are some possible causes of positive ELISA test.
Causes | Effects |
---|---|
HIV infection | Indicates the presence of HIV antibodies. |
Lyme disease exposure | Suggests prior exposure to Lyme disease bacteria. |
Syphilis infection | Signifies the presence of syphilis antibodies. |
Exposure to specific virus | Shows exposure or infection with the indicated virus. |
ELISA tests detect antibodies or antigens in blood, aiding in diagnosing various diseases and conditions.
Here are some possible causes of negative ELISA test.
Causes | Effects |
---|---|
Early-stage infection | May not have produced detectable antibodies yet. |
Immune response threshold | Antibody levels are below the test's detection limit. |
No exposure to the antigen | No antibodies present, indicating no prior exposure. |
Test limitations | False negatives can occur due to test sensitivity. |
ELISA test detects antibodies, with positive results indicating exposure or infection, while negatives suggest no or early exposure.
Here are the specimen requirements for the ELISA Test.
Specimen |
Blood or Saliva |
|
Volume |
1 ml |
|
Container |
Red-top tube or gel-barrier tube |
|
Storage |
Room temperature |
|
Sample Stability |
Temperature |
Period |
Room temperature |
14 days |
|
Refrigerated |
14 days |
|
Frozen |
14 days |
|
Freeze/thaw cycles |
Stable x4 |
|
Causes for Rejection |
Microbially-contaminated specimen; heat-treated specimen; specimens containing particulate matter; gross hemolysis; lipemia or icterus |
ELISA tests are generally safe and non-invasive.
Here is the basic limitation of ELISA Test.
Here are some of the risk factors of the ELISA Test.
Here are the Doctor's recommendations or consult a specialist after the ELISA Test.
Result | Doctor to Visit | Reason |
---|---|---|
Positive | Infectious Disease | Confirm diagnosis. |
Negative | Consulting Physician | Further evaluation. |
Here are the estimated ELISA Test Prices in India with different top cities:
City |
Price Range (INR)* |
500-1500 |
|
550-1500 |
|
500-1500 |
|
550-1500 |
|
500-1500 |
|
550-1500 |
|
500-1500 |
|
550-1500 |
|
550-1500 |
|
500-1500 |
|
550-1500 |
|
500-1500 |
|
550-1500 |
|
500-1500 |
*Prices are approximate and vary depending on a specific laboratory or healthcare facility.
Summary
Overall, ELISA Test, a versatile diagnostic tool, detects antibodies or antigens in blood samples, aiding in disease diagnosis and research. Also check Drlogy Test for detailed information about all medical tests for patients, doctors, scholers and medical students.
Reference
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