Dinitrophenylhydrazine or DNPH Test To Identify Aldehydes and Ketones

Dinitrophenylhydrazine or DNPH Test To Identify Aldehydes and Ketones

The DNPH test is a chemical test used to identify compounds with specific chemical groups, called ketones and aldehydes, in various substances.

 

DNPH Test

Here are the basic details of the DNPH Test.

Also Known As Brady's Reagent
Type Chemical Test
Purpose Detecting Ketone or Aldehyde Functional Groups
Sample Type Organic Compounds
Preparation Mixing DNPH Reagent with Ethanol
Fasting No
Gender No
Age Group No
Normal Value 0.6 mmol/L (millimoles per liter)
Reporting Time 192 hours
Cost 100-120

*Price range may vary as per location, lab type, and procedure of lab test.

 

DNPH Test Means

DNPH Test identifies ketones and aldehydes in chemical compounds.

  • Also known as Brady's reagent.
  • Forms dinitrophenylhydrazones indicating positive results.
  • Used in organic chemistry for functional group analysis.
  • Inexpensive and widely employed in research and analysis.



DNPH Test Purpose

The purpose of the DNPH Test is to:

  • DNPH Test detects ketone and aldehyde functional groups.
  • Identifies specific chemical compounds in organic substances.
  • Commonly used in chemical analysis and research.
  • Helps determine the presence of key functional groups.
  • Important in organic chemistry for structural characterization.
  • Forms dinitrophenylhydrazones as a positive indication.

 

DNPH Test Preparation

Here is the basic preparation for the DNPH Test.

 

Before Test

  • Prepare a sample containing the compound of interest.
  • Measure and collect the necessary reagents and equipment.

 

During Test

  • Mix the compound with DNPH reagent and ethanol.
  • Shake the mixture to facilitate reaction.
  • Observe for the formation of dinitrophenylhydrazone precipitate.

 

After Test

  • Interpret results based on the presence or absence of the precipitate.

 

DNPH Test Procedure

Here is the basic DNPH Test Procedure.

  • Mix compound with DNPH reagent and ethanol.
  • Shake the mixture to promote reaction.
  • Observe for dinitrophenylhydrazone precipitate formation.

 

DNPH Test Result

  • Positive result: Yellow (aliphatic) or orange/red (aromatic) precipitate.
  • Negative result: No color change or precipitate observed.
  • Interpretation based on presence or absence of precipitate.

 

DNPH Test Report

DNPH test report format example sample template- drlogy lab report

DNPH Test Report PDF

 

DNPH Test Positive Report

Dnph Positive Test Report Format Example Sample Template - Drlogy Pathology Lab Report Format

DNPH Positive Test Report PDF Format

DNPH Test Positive Result Report PDF

 

DNPH Test Negative Report

Dnph Negative Test Report Format Example Sample Template - Drlogy Pathology Lab Report Format

DNPH Negative Test Report PDF Format

DNPH Test Negative Result Report PDF

 

DNPH Test Normal Range

Here is the normal range of the DNPH Test.

DNPH Test Normal Value
DNPH 0.6 mmol/L (millimoles per liter)

 

DNPH Test Interpretation

Here is the Interpretation of the DNPH Test.

DNPH Test Result Interpretation
Positive Indicates the presence of ketones/aldehydes.
Negative Suggests the absence of ketones/aldehydes.

The DNPH test result is positive when a colored precipitate forms, indicating the presence of ketones or aldehydes, and negative when no precipitate forms, suggesting their absence.

 

DNPH Test Positive

Here are some possible causes of positive DNPH Test:

Possible Causes Effect
Ketones Presence of compounds with ketone functional groups.
Aldehydes Presence of compounds with aldehyde functional groups.
Carbonyl Compounds Detection of chemicals containing carbonyl (C=O) groups.

A positive DNPH test result is caused by the presence of ketones, aldehydes, or other carbonyl compounds in the tested substance.

 

DNPH Test Negative

Negative DNPH are less common and may not typically be a primary concern. However, here are some possible causes:

Possible Causes Effect
No Carbonyl Groups Absence of ketones, aldehydes, or carbonyl (C=O).
Incorrect Mixing Inadequate mixing of DNPH reagent with the sample.
Incomplete Reaction Insufficient reaction time for dinitrophenylhydrazone precipitate.

A negative DNPH test result typically occurs due to the absence of carbonyl groups in the tested substance, incomplete reactions, or incorrect mixing.

 

Specimen Requirements For DNPH Test

Here are the specimen requirements for the DNPH Test.

Specimen

Serum

Volume

1 ml

Container

Red-top tube or gel-barrier tube

Collection

Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping.

Storage

Refrigerate or freeze.

Sample Stability

Temperature

Period

Room temperature

7 day

Refrigerated

14 days

Frozen

60 days

Freeze/thaw cycles

Stable x1

Causes for Rejection

Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types

 

DNPH Test is Safe?

The DNPH test is generally safe when conducted following laboratory safety procedures.

  • Proper protective equipment (gloves, safety goggles) and ventilation should be used.
  • Hazardous chemical handling precautions must be taken.
  • Refer to safety guidelines and follow laboratory protocols.
  • Always exercise caution when working with chemicals.

 

When Do You Get DNPH Test Results?

  • DNPH test results are usually obtained immediately after the test.
  • Results are available as soon as the reaction is complete.
  • This typically takes only a few minutes after mixing the sample with the DNPH reagent.

 

DNPH Test Limitation

Here is the basic limitation of DNPH Test.

  • It primarily detects ketone and aldehyde functional groups, excluding other carbonyl compounds.
  • The test provides qualitative results but does not quantify the amount of ketones or aldehydes present.
  • Certain compounds may not react under standard conditions, leading to false negatives.
  • Some substances may interfere with the test, causing inaccurate results.
  • It is not suitable for detecting other functional groups commonly found in organic compounds.
  • The test may not work well with impure or complex mixtures, making interpretation challenging.

 

DNPH Test Risk Factors

Here are some of the risk factors of the DNPH Test.

  • Handling DNPH reagent involves exposure to potentially hazardous chemicals.
  • Inhalation of fumes or vapors may pose respiratory hazards.
  • Skin exposure can lead to irritation or allergic reactions.
  • Eye exposure may cause irritation or damage.
  • Ingesting DNPH reagent is toxic and must be avoided.

 

Doctor Recommendations After DNPH Test Result

 Here are the Doctor's recommendations or consult a specialist after the DNPH Test.

DNPH Test Result Doctor to Visit Reason
Positive Organic Chemist Identify and analyze specific carbonyl compounds.
Negative Not Applicable No medical doctor visit required, typically used in chemistry research.

DNPH Test, Positive result consults an organic chemist for compound analysis; negative result, no medical consultation needed, often used in research.

 

DNPH Test Price

Here are the estimated DNPH Test Prices in India with different top cities:

City

Price Range (INR)*

Mumbai

100-120

New Delhi

100-150

Bangalore

100-120

Hyderabad

100-150

Kolkata

100-120

Pune

100-150

Lucknow

100-120

Noida

100-150

Surat

100-120

Gurugram

100-120

Patna

100-150

Chennai

100-120

Jaipur

100-150

Ahmedabad

100-120

*Prices are approximate and vary depending on a specific laboratory or healthcare facility.

 

Summary

Overall, DNPH Test identifies ketones and aldehydes in chemicals; positive result shows their presence, while negative indicates their absence. Also check Drlogy Test for detailed information about all medical tests for patients, doctors, scholers and medical students.

 

Reference

  • 2,4-Dinitrophenylhydrazine - Wikipedia [1].
  • Application of 2,4-Dinitrophenylhydrazine (DNPH) in High ... - NIH [2].

 

favorite_border 5601 Likes

DNPH Test FAQ

What is the DNPH test?

The DNPH test, or 2,4-Dinitrophenylhydrazine test, is a chemical method used to detect and quantify carbonyl compounds in various substances. It's widely used in environmental, pharmaceutical, and industrial settings to analyze aldehydes and ketones.

Why is the DNPH test important?

The DNPH test is crucial because it helps identify and measure carbonyl compounds, which can be harmful pollutants, allergens, or important chemical intermediates. It plays a vital role in monitoring air quality, ensuring product quality, and assessing safety.

How does the DNPH test work?

The DNPH test involves reacting carbonyl compounds with 2,4-dinitrophenylhydrazine reagent to form stable derivatives. These derivatives can be easily measured using spectrophotometry or high-performance liquid chromatography (HPLC).

Is the DNPH test expensive?

The cost of the DNPH test depends on factors like the type of carbonyls being analyzed, sample volume, and the equipment used. While it may be relatively expensive for some applications, its accuracy justifies the investment.

INDIA’S MOST TRUSTED HEALTHCARE PLATFORM

10M+

Patients

22000+

Doctors

20000+

Hospitals/Labs

Drlogy Testclose

Top Test

Profile Test

Medical Condition

Sample Test

Test Category

Drlogy®
The Power To Health

Copyright © 2025 Drlogy. All rights reserved.