The DNPH test is a chemical test used to identify compounds with specific chemical groups, called ketones and aldehydes, in various substances.
Here are the basic details of the DNPH Test.
Also Known As | Brady's Reagent |
Type | Chemical Test |
Purpose | Detecting Ketone or Aldehyde Functional Groups |
Sample Type | Organic Compounds |
Preparation | Mixing DNPH Reagent with Ethanol |
Fasting | No |
Gender | No |
Age Group | No |
Normal Value | 0.6 mmol/L (millimoles per liter) |
Reporting Time | 192 hours |
Cost | 100-120 |
*Price range may vary as per location, lab type, and procedure of lab test.
DNPH Test identifies ketones and aldehydes in chemical compounds.
The purpose of the DNPH Test is to:
Here is the basic preparation for the DNPH Test.
Here is the basic DNPH Test Procedure.
DNPH Positive Test Report PDF Format
DNPH Negative Test Report PDF Format
Here is the normal range of the DNPH Test.
DNPH Test | Normal Value |
---|---|
DNPH | 0.6 mmol/L (millimoles per liter) |
Here is the Interpretation of the DNPH Test.
DNPH Test Result | Interpretation |
---|---|
Positive | Indicates the presence of ketones/aldehydes. |
Negative | Suggests the absence of ketones/aldehydes. |
The DNPH test result is positive when a colored precipitate forms, indicating the presence of ketones or aldehydes, and negative when no precipitate forms, suggesting their absence.
Here are some possible causes of positive DNPH Test:
Possible Causes | Effect |
---|---|
Ketones | Presence of compounds with ketone functional groups. |
Aldehydes | Presence of compounds with aldehyde functional groups. |
Carbonyl Compounds | Detection of chemicals containing carbonyl (C=O) groups. |
A positive DNPH test result is caused by the presence of ketones, aldehydes, or other carbonyl compounds in the tested substance.
Negative DNPH are less common and may not typically be a primary concern. However, here are some possible causes:
Possible Causes | Effect |
---|---|
No Carbonyl Groups | Absence of ketones, aldehydes, or carbonyl (C=O). |
Incorrect Mixing | Inadequate mixing of DNPH reagent with the sample. |
Incomplete Reaction | Insufficient reaction time for dinitrophenylhydrazone precipitate. |
A negative DNPH test result typically occurs due to the absence of carbonyl groups in the tested substance, incomplete reactions, or incorrect mixing.
Here are the specimen requirements for the DNPH Test.
Specimen |
Serum |
|
Volume |
1 ml |
|
Container |
Red-top tube or gel-barrier tube |
|
Collection |
Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping. |
|
Storage |
Refrigerate or freeze. |
|
Sample Stability |
Temperature |
Period |
Room temperature |
7 day |
|
Refrigerated |
14 days |
|
Frozen |
60 days |
|
Freeze/thaw cycles |
Stable x1 |
|
Causes for Rejection |
Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types |
The DNPH test is generally safe when conducted following laboratory safety procedures.
Here is the basic limitation of DNPH Test.
Here are some of the risk factors of the DNPH Test.
Here are the Doctor's recommendations or consult a specialist after the DNPH Test.
DNPH Test Result | Doctor to Visit | Reason |
---|---|---|
Positive | Organic Chemist | Identify and analyze specific carbonyl compounds. |
Negative | Not Applicable | No medical doctor visit required, typically used in chemistry research. |
DNPH Test, Positive result consults an organic chemist for compound analysis; negative result, no medical consultation needed, often used in research.
Here are the estimated DNPH Test Prices in India with different top cities:
City |
Price Range (INR)* |
100-120 |
|
100-150 |
|
100-120 |
|
100-150 |
|
100-120 |
|
100-150 |
|
100-120 |
|
100-150 |
|
100-120 |
|
100-120 |
|
100-150 |
|
100-120 |
|
100-150 |
|
100-120 |
*Prices are approximate and vary depending on a specific laboratory or healthcare facility.
Summary
Overall, DNPH Test identifies ketones and aldehydes in chemicals; positive result shows their presence, while negative indicates their absence. Also check Drlogy Test for detailed information about all medical tests for patients, doctors, scholers and medical students.
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